“Formation and function of 3D chromatin domains for gene regulation"

Date

Jan 19, 2023

Time

11:00 AM - 12:00 PM

Speaker

Daniel Ibrahim

Affiliation

Max Planck Institute for Molecular Genetics, Berlin

Series

MPI-CBG Thursday Seminar

Language

en

Main Topic

Biologie

Host

Jesse Veenvliet

Description

Enhancers can regulate their target promoters over large genomic distances for which spatial proximity between these elements is assumed to be necessary. Topologically Associating Domains (TADs) are thought to provide a spatial environment in the nucleus, harboring multiple enhancers and are thus related to gene regulation. However, to which degree TADs contribute to gene regulation and how enhancer position within the TAD affects its function in vivo is not known. I will present work that uses genome-engineering to generate a series of mouse lines with alleles designed to test how important TADs are for gene regulation. At well-characterized developmental loci, we either re-arrange TAD boundaries or reposition individual enhancers and analyze the effects on 3D chromatin structure, gene expression and mouse phenotype in vivo. Thereby we can show that TADs provide a supportive environment for enhancer function but also point to our limited understanding of the interplay between regulatory elements.

Last modified: Jan 20, 2023, 7:35:47 AM