Bi

In situ single-molecule dynamics of the SOS-repressor LexA during antibiotic stress

Date
Oct 15, 2020
Time
11:00 AM - 12:00 PM
Speaker
Michael Schlierf
Affiliation
Technische Universität Dresden, Center for Molecular and Cellular Bioengineering (CMCB) B CUBE, Germany
Series
MPI-CBG Thursday Seminar
Language
en
Main Topic
Biologie
Host
Stefan Diez
Description
In bacteria, the key mechanism facilitating survival, mutation and adaptation upon DNA damage is the SOS response. Through autoproteolytic digestion triggered by single-stranded DNA, the repressor LexA controls over 50 SOS genes. Efforts to inhibit this response and thereby combat antibiotic resistance rely on a broad understanding of its behavior in vivo, which is still limited. Here, we use single-molecule localization microscopy to directly visualize LexA in Escherichia coli. With single-particle tracking PALM, we observe the repressor under antibiotic stress of different intensity and identify four diffusive populations. Using a set of LexA mutants, we assign operator bound, target-searching, freely diffusing and cleaved repressors. Measuring their time-evolution and applying a kinetic model, we obtain in vivo cleavage and binding rates in dependence of antibiotic dosage. A strong SOS response is even observed at low antibiotic stress. We further develop a strategy to count molecules in fixed cells and found that LexA is much more abundant during the late SOS response than previously thought.

Last modified: Oct 16, 2020, 12:09:58 AM

Location

Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG Auditorium (big half))Pfotenhauerstraße10801307Dresden
Phone
+49 351 210-0
Fax
+49 351 210-2000
E-Mail
MPI-CBG
Homepage
http://www.mpi-cbg.de

Organizer

Max Planck Institute of Molecular Cell Biology and GeneticsPfotenhauerstraße10801307Dresden
Phone
+49 351 210-0
Fax
+49 351 210-2000
E-Mail
MPI-CBG
Homepage
http://www.mpi-cbg.de
Scan this code with your smartphone and get directly this event in your calendar. Increase the image size by clicking on the QR-Code if you have problems to scan it.
  • BiBiology
  • ChChemistry
  • CiCivil Eng., Architecture
  • CoComputer Science
  • EcEconomics
  • ElElectrical and Computer Eng.
  • EnEnvironmental Sciences
  • Sfor Pupils
  • LaLaw
  • CuLinguistics, Literature and Culture
  • MtMaterials
  • MaMathematics
  • McMechanical Engineering
  • MeMedicine
  • PhPhysics
  • PsPsychology
  • SoSociety, Philosophy, Education
  • SpSpin-off/Transfer
  • TrTraffic
  • TgTraining
  • WlWelcome