Immobilistion and Controlled Activation of Peptide and Proteins

Datum

14.08.2015

Zeit

13:00 - 14:00

Sprecher

Prof. Annette Beck-Sickinger

Zugehörigkeit

Universität Leipzig

Sprache

en

Hauptthema

Materialien

Andere Themen

Materialien, Physik

Host

Ulrike Steere

Beschreibung

Immobilisation and Controlled Activation of Peptide and Proteins Institute of Biochemistry, Faculty of Biosciences, Pharmacy and Psychology, Leipzig University, Brüderstr. 34, 04103 Leipzig, Germany Multifunctionality is gaining more and more importance in the field of improved biomaterials. Accordingly, specific and selective modification of surfaces is required. Peptides feature a broad chemical variability and are versatile mediators between inorganic surfaces or polymers and living cells. The fully biodegradable polymer poly(lactic-co-glycolic acid) (PLGA) is frequently used for biomedical implants and can be applied as nanoparticles for drug delivery . On the other side inorganic surfaces, e. g. titanium oxide or silicium oxide are frequently used inorganic materials that require specific attachment of cells. We developed several strategies for immobilization of peptides and proteins on hydrophobic, hydrophilic and inorganic surfaces using selectively binding peptides or bioorthogonal ligation and click- or click-like reactions. In the next step, we developed linkers for controlled release of therapeutics, e. g. chemokines like interleukin 8 or stromal derived factor 1 alpha (SDF-1 alpha) , which has been shown to promote and initiate migration of progenitor cells. Chemokines were produced recombinantly and chemically modified by native chemical ligation strategy . Peptide linkers were obtained by solid phase peptide synthesis. By the combination of different strategies we could induce cell spreading and cell viability of osteoblasts on inorganic surfaces , migration of eEPCs towards hydrogel slices and activation of chemokines by light . Accordingly, novel types of biomaterials with significantly improved surfaces have been developed.

Links

• Seminars at the IFW

Letztmalig verändert: 14.08.2015, 09:57:28