Bi

C-terminal tail of tubulin mediate assembly of flagellar microtubule doublet in vitro

Datum
05.03.2019 
Zeit
11:00 Uhr - 12:00 Uhr 
Sprecher
Marketa Cernohorska 
Institut
Institute of Molecular Genetics of the ASCR, v. v. i., Prague, Czech Republic 
Sprache
en 
Hauptthema
Biologie: Zellbiologie (incl. Molekulare), Entwicklungsbiologie
Ansprechpartner
Gaia Pigino 
Zusammenfassung

In centriole, microtubules are assembled in conserved structure of nine-fold blades in triplets (A, B, C-microtubule) that elongates as doublets (A, B-microtubule) to the axoneme and provide scaffold for intraflagellar transport. While tough structure of these blades has long been known, the mechanism by which they form remained unknown. The most recent work on flagellar doublets using cryo-electron tomography (Ichikawa et al., 2017) suggested two possible hypotheses. First, B-microtubule is bound to A-microtubule via small microtubule inner proteins penetrating through the microtubule wall, and second, both microtubules might be bound directly via unique side-to-side contacts. In order to reveal the nature and mechanism of microtubule doublet assembly,
I employed a cell-free assay combined with limited proteolytic digestion. This approach showed for the first time that microtubule doublet is assembled solely from tubulin. In this talk I will explain our discovery with Paul Guichard and Virginie Hamel of the inhibitory role of tubulin C-terminus. In addition, I will show my recent project which aims to reveal BBSome assembly using novel technique of expansion microscopy.

 

Letzte Aktualisierung: 06.03.2019 00:06.

Vortragsort 

Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG Galleria) 
Pfotenhauerstraße 108
01307 Dresden
Telefon
+49 351 210-0 
Fax
+49 351 210-2000 
E-Mail
Max Planck Institute of Molecular Cell Biology and Genetics 
Homepage
http://www.mpi-cbg.de 

Veranstalter 

Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG)
Pfotenhauerstraße 108
01307 Dresden
Telefon
+49 351 210-0 
Fax
+49 351 210-2000 
E-Mail
Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG) 
Homepage
http://www.mpi-cbg.de 
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