Bi

Trans-endocytosis from filopodia: How ephrins surf between cells to be remembered

Datum
08.02.2019 
Zeit
11:00 Uhr - 12:00 Uhr 
Sprecher
José Ignacio Valenzuela Iturra 
Institut
Institut Curie, CNRS - UMR144, Paris, France 
Sprache
en 
Hauptthema
Biologie: Zellbiologie (incl. Molekulare), Entwicklungsbiologie
Ansprechpartner
Marino Zerial 
Zusammenfassung

Ephrins can elicit either contact-mediated cell-cell adhesion or repulsion depending on the efficiency of removal of their ligand/receptor complexes from the cell surface, thus controlling tissue morphogenesis and oncogenic development. However, the dynamic of turnover of the newly assembled ephrin/Eph-receptor complexes during cell-cell interactions remains mostly unexplored. By using the RUSH (Retention Using Selective Hooks) system, we show that ephrin-A1/EphA2 complexes are locally formed at the tip of cytoneme-like filopodia, at cell-to-cell contacts. Clusters of ephrin-A1 from donor cells surf on filopodia associated to EphA2-bearing sub-domains of acceptor cells. Full-length ephrin-A1 is transferred to acceptor cells by trans-endocytosis through a proteolysis-independent mechanism. Trans-endocytosed ephrin-A1 bound to its receptor enables signaling to be emitted from endo-lysosomes of acceptor cells even after cell division. Localized trans-endocytosis of ephrin-A1 sustains contact-mediated repulsion on cancer cells. Our results uncover the essential role played by local concentration at the tip of filopodia and the trans-endocytosis of full-length ephrin to maintain a memory of ephrin signaling.

 

Letzte Aktualisierung: 09.02.2019 00:07.

Vortragsort 

Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG Seminar Room 4th Floor) 
Pfotenhauerstraße 108
01307 Dresden
Telefon
+49 351 210-0 
Fax
+49 351 210-2000 
E-Mail
Max Planck Institute of Molecular Cell Biology and Genetics 
Homepage
http://www.mpi-cbg.de 

Veranstalter 

Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG)
Pfotenhauerstraße 108
01307 Dresden
Telefon
+49 351 210-0 
Fax
+49 351 210-2000 
E-Mail
Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG) 
Homepage
http://www.mpi-cbg.de 
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