Bi

Integrating Information from Diverse Microscope Images: Learning and Using Generative Models of Cell Organization

Datum
12.03.2019 
Zeit
18:00 Uhr - 19:00 Uhr 
Sprecher
Robert F. Murphy 
Institut
Carnegie Mellon University, Pittsburgh, USA 
Sprache
en 
Hauptthema
Biologie: Zellbiologie (incl. Molekulare), Entwicklungsbiologie
Ansprechpartner
Ivo Sbalzarini 
Zusammenfassung

Systems biology efforts require accurate, cell-type specific information about the shape and distributions of subcellular structures and the distributions of proteins and other macromolecules in order to be able to capture and simulate cellular spatiotemporal dynamics. We have developed tools to build generative models of cell organization directly from microscope images of many cells. Our open source system, CellOrganizer (http://CellOrganizer.org), contains components that can build probabilistic generative models of cell, nucleus, organelle and protein distributions. The models also capture heterogeneity within cell populations. We have recently addressed two critical challenges in constructing these models. The first is to choose appropriate methods for generating cell, nucleus or organelle shapes. We have recently evaluated a number of generative methods with respect to their reconstruction accuracy: how well they can capture/represent a complicated shape. The results show that spherical harmonic methods provide the best reconstructions and most reasonable synthetic shapes. The second challenge is to be able to learn the dependence of component distributions upon each other, i.e., the spatial relationships between different components. As these relationships are learned, generative models can be created from images of different proteins and organelles and then combined to create synthetic cells having many more components than can be imaged together. We have explored approaches for inferring spatial relationships between components without directly measuring them. An important use for the resulting models of cell organization is to generate synthetic cell shapes and organelle distributions that can be used as geometries for cell simulations. This permits a structured exploration of the dependencies of cellular biochemistry upon cell morphology and organization.

 

Letzte Aktualisierung: 13.03.2019 00:06.

Vortragsort 

Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG Auditorium (small half) 
Pfotenhauerstraße 108
01307 Dresden
Telefon
+49 351 210-0 
Fax
+49 351 210-2000 
E-Mail
Max Planck Institute of Molecular Cell Biology and Genetics 
Homepage
http://www.mpi-cbg.de 

Veranstalter 

Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG)
Pfotenhauerstraße 108
01307 Dresden
Telefon
+49 351 210-0 
Fax
+49 351 210-2000 
E-Mail
Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG) 
Homepage
http://www.mpi-cbg.de 
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