Bi

Defining the identity and dynamics of adult gastric isthmus stem cells

Datum
08.01.2019 
Zeit
11:00 Uhr - 12:00 Uhr 
Sprecher
Bon-Kyoung Koo 
Institut
IMBA, Vienna, Austria 
Sprache
en 
Hauptthema
Biologie: Zellbiologie (incl. Molekulare), Entwicklungsbiologie
Ansprechpartner
Anne Grapin-Botton 
Zusammenfassung

The gastric corpus epithelium is the thickest part of the gastro-intestinal tract and is characterized by rapid tissue turnover. Several markers have been proposed for gastric corpus stem cells in both isthmus and base regions. However, the identity of isthmus stem cells (IsthSCs) and the interaction between distinct stem cell populations is still under debate. Here, based on unbiased genetic labeling and biophysical modeling, we show that corpus glands are compartmentalized into two independent zones, with actively-cycling stem cells maintaining the pit-isthmus-neck region and slow-cycling reserve stem cells maintaining the base. Independent lineage tracing based on Stmn1 and Ki67 expression confirmed that rapidly-cycling IsthSCs maintain the pit-isthmus-neck of corpus glands. Finally, single cell RNA-seq analysis is used to define the molecular identity and lineage relationship of a single, cycling IsthSC population. These observations define the identity and functional behavior of a single population of actively-cycling IsthSCs.

 

Letzte Aktualisierung: 09.01.2019 00:06.

Vortragsort 

Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG Auditorium (big half)) 
Pfotenhauerstraße 108
01307 Dresden
Telefon
+49 351 210-0 
Fax
+49 351 210-2000 
E-Mail
Max Planck Institute of Molecular Cell Biology and Genetics 
Homepage
http://www.mpi-cbg.de 

Veranstalter 

Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG)
Pfotenhauerstraße 108
01307 Dresden
Telefon
+49 351 210-0 
Fax
+49 351 210-2000 
E-Mail
Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG) 
Homepage
http://www.mpi-cbg.de 
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