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The deadly touch: protein denaturation at the air-water interface and how to prevent it

date
21.01.2019 
time
11:00 AM - 12:00 PM 
speaker
Edoardo D’Imprima 
affiliation
Structural Biology Department, Max Planck Institute of Biophysics, Frankfurt 
language
en 
main topic
Biology: Cell Biology (incl. Molecular, Structural), Developmental Biology
host
Gaia Pigino 
abstract

Electron cryo-microscopy analyzes the structure of proteins and protein complexes in vitrified solution. Proteins tend to be adsorbed to the air-water interface in unsupported film of aqueous solution, which can result in partial or complete denaturation of the macromolecular complex. We investigated the structure of Saccharomyces cerevisiae fatty acid synthase (FAS) at the air-water interface by electron cryo-tomography and single particle image processing. Around 90% of complexes adsorbed to the air-water interface are partly denatured. Combining our results in cryo-conditions with negative stain experiments we demonstrate that denaturation by contact with air may happen at any stage of specimen preparation. Denaturation at the air-water interface is completely avoided when the complex is plunge-frozen on a substrate of hydrophilized graphene.

 

Last update: 21.01.2019 00:08.

venue 

Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG Galleria) 
Pfotenhauerstraße 108
01307 Dresden
telefon
+49 351 210-0 
fax
+49 351 210-2000 
e-mail
Max Planck Institute of Molecular Cell Biology and Genetics 
homepage
http://www.mpi-cbg.de 

organizer 

Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG)
Pfotenhauerstraße 108
01307 Dresden
telefon
+49 351 210-0 
fax
+49 351 210-2000 
e-mail
Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG) 
homepage
http://www.mpi-cbg.de 
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