Single-Molecule Spectroscopy of Intrinsically Disordered Proteins and Their Interaction Mechanisms

04:00 PM - 05:00 PM 
Ben Schuler 
University of Zurich, Institute of Biochemistry 
part of series
BIOTEC Green Seminar 
main topic
Biology: general
Medicine: Regeneration
Michael Schlierf / James Saenz 

AbstractThe functions of proteins have traditionally been linked to their well-defined three-dimensional, folded structures. It is becoming increasingly clear, however, that many proteins perform essential functions without being folded. Single-molecule spectroscopy provides new opportunities for investigating the structure, dynamics, and interaction mechanisms of such unfolded or 'intrinsically disordered' proteins (IDPs). I will focus on a recent example of two IDPs that bind one another with very high affinity yet remain unstructured. This type of interaction has interesting ramifications for kinetic mechanisms of binding and cellular regulation.
5 most recent papersBorgia, A., Borgia, M., Bugge, K., Kissling, V.M., Heidarsson, P.O., Fernandes, C.B., Sottini, A., Soranno, A., Buholzer, K., Nettels, D., Kragelund, B.B., Best, R.B., & Schuler, B. (2018) Extreme disorder in an ultra-high-affinity protein complex. Nature, 555, 61-66.
Zosel, F., Mercadante, D., Nettels, D. & Schuler, B. (2018) A proline switch explains kinetic heterogeneity in a coupled folding and binding reaction. Nat. Comm. 9:3332
Soranno, A., Holla, A., Dingfelder, F., Nettels, D., Makarov, D.E. & Schuler, B. (2017) Integrated view of internal friction in unfolded proteins from single-molecule FRET, contact quenching, theory, and simulations. Proc. Natl. Acad. Sci. USA 114, E1833-E1839.
Schuler, B. (2018) Perspective: Chain dynamics of unfolded and intrinsically disordered proteins from nanosecond fluorescence correlation spectroscopy combined with single-molecule FRET. J. Chem. Phys. 149, 010901
Schuler, B., Hofmann, H., Nettels, D. & Soranno, A. (2016) Single-molecule FRET spectroscopy and the polymer physics of unfolded and intrinsically disordered proteins. Annu. Rev. Biophys. 45, 207-231.


Last update: 25.10.2018 00:09.


DFG Center for Regenerative Therapies Dresden (CRTD, auditorium left) 
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DFG Center for Regenerative Therapies Dresden 


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