BiMe

Decoding lineage emergence in the lateral plate mesoderm

date
22.10.2018 
time
01:00 PM - 02:00 PM 
speaker
Christian Mosimann 
affiliation
University of Zurich, Institute of Molecular Life Sciences 
part of series
BIOTEC Green Seminar 
language
en 
main topic
Biology: general
subtopics
Medicine: Regeneration
host
Dr. Nikolay Ninov 
abstract

AbstractCell fate control from multipotent progenitor cells of key importance to understanding organ formation, origins of congenital defects, and regeneration. We study the emergence of cardiovascular cell fates within the context of mesoderm patterning with cellular, molecular, and evolutionary approaches using zebrafish as principal model. We have uncovered gene-regulatory elements that reveal the developmental stages from earliest cardiovascular prongenitor formation to subsequent organ patterning. Decoding the upstream input for these regulatory elements, we uncovered an ancient molecular program that drives the formation of cardiovascular-competent mesoderm. Our approaches further instruct the discovery and mechanistic follow-up of genotype-phenotype associations in congential cardiovascular diseases.
5 most recent papers1) Felker A, Prummel KD, Merks AM, Mickoleit M, Brombacher EC, Huisken J, Panáková D, Mosimann C. Continuous addition of progenitors forms the cardiac ventricle in zebrafish. Nat Commun. 2018 May 21;9(1):2001. doi: 10.1038/s41467-018-04402-6.
2) Cantù C, Felker A, Zimmerli D, Prummel KD, Cabello EM, Chiavacci E, et al.. Mutations in Bcl9 and Pygo genes cause congenital heart defects by tissue-specific perturbation of Wnt/beta-catenin signaling Genes Dev. accepted, in print.
3) Maximizing mutagenesis with solubilized CRISPR-Cas9 ribonucleoprotein complexes. Burger A, Lindsay H, Felker A, Hess C, Anders C, Chiavacci E, Zaugg J, Weber LM, Catena R, Jinek M, Robinson MD, Mosimann C. Development. 2016 Jun 1;143(11):2025-37. doi: 10.1242/dev.134809. Epub 2016 Apr 29.
4) Lindsay H, Burger A, Biyong B, Felker A, Hess C, Zaugg J, Chiavacci E, Anders C, Jinek M, Mosimann C, Robinson MD. CrispRVariants charts the mutation spectrum of genome engineering experiments. Nat Biotechnol. 2016 Jul 12;34(7):701-2. doi: 10.1038/nbt.3628.
5) Mosimann C, Panáková D, Werdich AA, Musso G, Burger A, et al. Chamber identity programs drive early functional partitioning of the heart. Nat Commun. 2015 Aug 26;6:8146. doi: 10.1038/ncomms9146.

 

Last update: 22.10.2018 00:05.

venue 

DFG Center for Regenerative Therapies Dresden (CRTD, auditorium left) 
Fetscherstraße 105
01307 Dresden
telefon
+49 (0)351 458 82064 
fax
+49 (0)351 458 82059  
e-mail
DFG Center for Regenerative Therapies Dresden 
homepage
http://www.crt-dresden.de 

organizer 

DFG Center for Regenerative Therapies Dresden (CRTD)
Fetscherstraße 105
01307 Dresden
telefon
+49 (0)351 458 82064 
fax
+49 (0)351 458 82059 
e-mail
DFG Center for Regenerative Therapies Dresden (CRTD) 
homepage
http://www.crt-dresden.de 
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