Single molecule detection for in vitro diagnostics

Date

Jul 23, 2015

Time

2:30 PM - 4:00 PM

Speaker

Prof. Dr. Thomas Kirner

Affiliation

Fachhochschule Hamm

Series

IMB - Seminar

Language

en

Main Topic

Biologie

Other Topics

Biologie

Host

IMB

Description

A highly sensitive detection system for diagnostic applications will be presented. The system is designed to meet the needs of medical diagnostics for reliable measurements of pathogens and biomarkers in the low concentration regime. It consists of a confocal detection unit, micro-structured sampling cells, and a “Virtual lab” analysis software. The detection unit works with laser induced fluorescence and is designed to provide accurate and highly sensitive measurement at the single molecule level. Various sampling cells are micro-structured in glass, silicon or polymers to enable measurements under flow and nonflow conditions. Sampling volume is below one microliter. The „“Virtual lab” software analyzes the light intensity online according to the patent pending “Accurate Stochastic Fluorescence Spectroscopy” (ASFS) developed by FluIT Biosystems GmbH. Tools for simulation and experiment optimization are included as well. Experimental results for various applications with relevance for in vitro diagnostics will be presented.

Last modified: Jul 15, 2015, 2:38:17 PM