Advanced cryo-electron tomography for visualization of supramolecular organizations of 70S ribosomes inside E. coli cells

Date

Mar 18, 2015

Time

11:00 AM - 12:00 PM

Speaker

Julio Ortiz

Affiliation

Max Planck Institute of Biochemistry, Martinsried, Germany

Language

en

Main Topic

Biologie

Other Topics

Biologie

Host

Gaia Pigino

Description

Julio Ortiz is an expert in cryo-electron tomography (CET) and image processing. In particular, he applies these techniques to the structural description of macromolecular complexes in complex systems, as lysates or whole cells. In the last decade, his work has been focused on the analysis of 70S ribosomes supramolecular organizations, such as polysomes or the resting ribosome pairs (100S). The main interest of his research is to reveal biologically significant molecular associations occurring in the native environment of the cell. In this sense, he has contributed to a series of recent developments in the area of cryo-electron microscopy that can be applied to cells to gain information of their molecular inventory. In his talk, Dr. Ortiz will present the basic principles of CET and associated techniques, i.e. the Focused Ion Beam technology (FIB), which allows the application of CET to thick specimens. New insights into the 3D organization of ribosomes in E.coli will be presented as well.

Last modified: Mar 19, 2015, 9:04:30 AM