High-Throughput Single-Molecule Analysis of DNA-Protein Interactions Using DNA Curtains

Date

Mar 9, 2026

Time

2:00 PM - 3:00 PM

Speaker

Emanuel N. Lissek

Affiliation

1NA

Language

en

Main Topic

Biologie

Host

LMF

Description

DNA curtains are a high-throughput single-molecule assay that enables simultaneous visualization of thousands of DNA-protein interactions, revealing statistically relevant single-molecule level insights. DNA molecules are aligned on nanofabricated barriers within a microfluidics flow cell, allowing real-time observation of dynamic processes such as DNA replication, and repair, using TIRF microscopy and/or spinning disk confocal microscopy. 1NA's DNA curtains flow cell addresses key barriers of implementation by applying semiconductor manufacturing practices to the nanofabrication of the barriers. Flow cell design minimizes dead volume and reagent use, while protocols have been optimized for rapid setup. This approach preserves the resolution of single-molecule methods while overcoming throughput limitations. It is particularly well-suited for in vitro studies of DNA-binding proteins or the mechanistic characterization of small-molecule modulators. Applications include quantifying DNA-protein kinetics, mapping interaction heterogeneity, or screening small molecule interactions.

Last modified: Feb 27, 2026, 7:37:53 AM