A reconstituted mammalian APC-kinesin complex selectively transports defined packages of axonal mRNAs

Date

Feb 26, 2020

Time

11:00 AM - 12:00 PM

Speaker

Sebastian Maurer

Affiliation

CRG Barcelona, Spain

Language

en

Main Topic

Biologie

Host

Jan Brugues

Description

Through the asymmetric distribution of mRNAs cells spatially regulate gene expression to create cytoplasmic domains with specialized functions. In neurons, mRNA localization is required for essential processes such as cell polarization, migration and synaptic plasticity underlying long-term memory formation. We report the first reconstitution of a mammalian mRNA transport system revealing that the tumour suppressor adenomatous polyposis coli (APC) forms stable complexes with the axonally localized b-actin and b2B-tubulin mRNAs which are linked to a kinesin-2 via the cargo adaptor KAP3. APC activates kinesin-2 and both proteins are sufficient to drive specific transport of defined mRNA packages. Guanine-rich sequences located in 3’UTRs of axonal mRNAs increase transport efficiency and balance the access of different mRNAs to the transport system. Our findings reveal for the first time a minimal set of proteins sufficient to transport mammalian mRNAs.

Last modified: Feb 27, 2020, 12:07:01 AM