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UID:DSC-16921
DTSTART;TZID=Europe/Berlin:20200203T110000
SEQUENCE:1580771186
TRANSP:OPAQUE
DTEND;TZID=Europe/Berlin:20200203T120000
URL:https://www.dresden-science-calendar.de/calendar/en/detail/16921
LOCATION:MPI-CBG\, Pfotenhauerstraße 10801307 Dresden
SUMMARY:Marco: Towards high-throughput in-situ structural biology
CLASS:PUBLIC
DESCRIPTION:Speaker: Alex de Marco\nInstitute of Speaker: Monash University
 \, Dept. of Biochemistry and Molecular Biology\, ARC Centre of Excellence 
 in Advanced Molecular Imaging\, Clayton\, Australia\nTopics:\n\n Location:
 \n  Name: MPI-CBG (MPI-CBG Galleria)\n  Street: Pfotenhauerstraße 108\n  
 City: 01307 Dresden\n  Phone: +49 351 210-0\n  Fax: +49 351 210-2000\nDesc
 ription: Cryo-transmission electron tomography (cryoET) in association wit
 h cryo-focused ion beam (cryoFIB) milling enables structural biology studi
 es to be performed directly within the cellular environment. Cryo-preserve
 d cells are milled and a lamella with a thickness of 200-300 nm provides a
 n electron transparent window suitable for cryoET imaging. CryoFIB milling
  is an effective method\, but it is a tedious and time-consuming process\,
  which typically results in ~10 lamellae per day. Here\, we introduce an a
 utomated method to reproducibly prepare cryo-lamellae on a grid and reduce
  the amount of human supervision throughout the process. We tested the rou
 tine on cryo-preserved Saccharomyces cerevisiae and demonstrate that it is
  possible to increase the throughput and achieve a rate of 5 lamellae/hour
  without the need to supervise the preparation. We demonstrate that the qu
 ality of the lamellae is consistent throughout the preparation and their c
 ompatibility with cryoET analyses. This method in association with the int
 egration of a cryo-light microscope inside the cryoFIB and with the optimi
 zation of the ion source leads to the possibility to prepare efficiently t
 argeted lamellae in tissue.
DTSTAMP:20261111T164247Z
CREATED:20200114T230827Z
LAST-MODIFIED:20200203T230626Z
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