Bone cell differentiation in tubular micro scaffolds

Date

Mar 29, 2018

Time

10:00 AM - 11:00 AM

Speaker

Raffael Herzer

Affiliation

IFW Dresden (IIN)

Language

en

Main Topic

Materialien

Other Topics

Materialien, Physik

Host

Kristina Krummer-Meier

Description

As a consequence of the rising age of the world population, the number of bone fractures and age related diseases like osteoporosis is increasing [1]. Therefore, recent research focuses on the development of new implant materials that can replace the commonly used Ti alloys (cp(2)-Ti, Ti6Al4V), which release toxic agents into the human body [2]. In that respect, pure beta – phase materials like TiNb alloys are promising candidates. They show superior properties like a low Young’s modulus, high corrosion resistance and low toxicity [3]. However, to improve them further, a deeper understanding of the interplay between bone cells and the implanted material is required. So far, the exact mechanism of the implant osseointegration and its long-term biocompatibility remains unknown. Besides, recent studies with individual cells showed that single cells behave differently than a whole bulk cell population. Furthermore, the attention is centered on 3D confinements which play a crucial role in the cell behavior. In that respect, our group showed that the cell migration and mitosis is highly influenced by the 3D confinement and the cell morphology was closer to the observed in in vivo conditions [4]. Building up on this previous work, we want to take advantage of the roll-up technology to create a 3D platform to analyze the interactions of individual bone cells with the implant material surface. The first steps in this direction were taken by transferring the TiNb alloy onto a planar substrate to characterize the material composition after using different metal deposition techniques. In a second step, 3D tubular scaffolds were fabricated using a combination of biocompatible materials through strain-engineering. Finally, we established in our laboratory a bone cell culture using MSC (mesenchymal stem cells) which play an important role in the bone healing process and successfully differentiated them into bone forming cells. Such a platform will serve to study the effects of surface modifications on the morphology and function of living bone cells as well as to study the influence of different stimuli (e.g. chemical, physical) in their differentiation process. [1] L. Hoesel et al. Medical Science Monitor 14.3 (2008): HY1-HY8. [2] Y. Li et al. Materials 7.3 (2014): 1709-1800. [3] M. Geetha et al. Progress in materials science 54.3 (2009): 397-425. [4] S. Weiz et al. Advanced Biosystems 2.2 (2017): 1-32.

Links

• Seminars at the IFW

Last modified: Mar 29, 2018, 9:52:50 AM