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UID:DSC-22605
DTSTART;TZID=Europe/Berlin:20260126T111500
SEQUENCE:1769409419
TRANSP:OPAQUE
DTEND;TZID=Europe/Berlin:20260126T121500
URL:https://www.dresden-science-calendar.de/calendar/de/detail/22605
LOCATION:MPI-CBG\, Pfotenhauerstraße 10801307 Dresden
SUMMARY:Sandoghdar: Cryogenic microscopy of protein complexes at angstrom r
 esolution &amp\; three-dimensional fluorescence-free imaging and tracking 
 in live cells
CLASS:PUBLIC
DESCRIPTION:Speaker: Vahid Sandoghdar\nInstitute of Speaker: Max Planck Ins
 titute for the Science of Light &amp\; Max-Planck-Zentrum für Physik und 
 Medizin Erlangen\, Germany\nTopics:\n\n Location:\n  Name: MPI-CBG (MPI-CB
 G CSBD SR Top Floor (VC))\n  Street: Pfotenhauerstraße 108\n  City: 01307
  Dresden\n  Phone: +49 351 210-0\n  Fax: +49 351 210-2000\nDescription: Fl
 uorescence microscopy has been the workhorse of biological microscopy in t
 he last half a century\, also leading to super-resolution microscopy. In t
 he first part of this presentation\, I will discuss the application of cry
 ogenic fluorescence microscopy for improving the photophysics of fluoropho
 res\, thus\, allowing us to reach three-dimensional angstrom resolution in
  resolving several sites of a single protein or protein complex. While flu
 orescence labeling remains a powerful tool\, it poses fundamental limitati
 ons\, which have motivated many groups to develop fluorescence-free measur
 ement methods. Among various contrast mechanisms\, scattering offers uniqu
 e opportunities. About two decades ago\, we showed that single gold nanopa
 rticles as small as 5 nm could be detected via interferometric detection o
 f their scattering\, coined iSCAT. Since then\, it has been shown that unl
 abeled nano-objects such as proteins and viruses as small as 10 kDa can be
  detected\, counted and tracked. We will discuss the application of this m
 ethod in characterizing extracellular vesicles and cellular secretomes. Fu
 rthermore\, I will present our most recent results on three-dimensional la
 bel-free imaging of cellular events such as the endoplasmic reticulum and 
 microtubule dynamics as well as virus diffusion via confocal iSCAT microsc
 opy. In addition to our iSCAT work\, I plan to present an efficient method
  for delivery of nanoparticles and small molecules to well-defined positio
 ns on a cell.
DTSTAMP:20260818T100001Z
CREATED:20260122T063859Z
LAST-MODIFIED:20260126T063659Z
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