Microstructured scaffolds for muscle tissue engineering and their effect on cell organization, myogenic gene expression, and transcriptomic profiles

Datum

24.10.2024

Zeit

11:00 - 12:00

Sprecher

Dragica Bezjak

Zugehörigkeit

Universidad Técnica Federico Santa María, Valparaíso, Chile

Serie

MPI-CBG Thursday Seminar

Sprache

en

Hauptthema

Biologie

Host

Meritxell Huch

Beschreibung

Muscle tissue engineering holds significant promise for regenerative medicine, disease modeling, and cultured meat production. This field utilizes muscle cells, scaffolds to support these cells, and biochemical cues to guide their development. For muscle cells to mature into functional tissue, they must undergo myogenesis, which involves cell proliferation, followed by exit from cell cycle, differentiation, alignment, and fusion into elongated, multinucleated myofibers. An essential aspect of effective scaffold design is creating structures that promote proper cell alignment and fusion. In this study, we fabricated scaffolds from marine-derived biopolymers, including salmon gelatin, alginate, agarose, and glycerol, resulting in both flat and microstructured surfaces for comparative analysis. We assessed the impact of these microstructured scaffolds on muscle cell behavior, focusing on alignment, glycolytic metabolism, myogenic gene expression, and transcriptomic profiles. Muscle cells cultured on microchannel scaffolds developed parallel, elongated, multinucleated structures resembling muscle bundles, with elevated glycolytic metabolism, when compared to the use of flat scaffolds. Gene expression analysis revealed significant correlations between myogenic and fusion markers, such as Myomaker with MyoD, Myomixer with Myosin heavy chain, and Myogenin with Myosin heavy chain. Immunofluorescence confirmed the expression of these markers after seven days of culture. Transcriptome analysis using high throughput sequencing highlighted notable differences between flat and microstructured scaffolds. Functional enrichment analysis identified key gene modules related to muscle development, including filament sliding, muscle contraction, and sarcomere organization. This study enhances the understanding of scaffold design in muscle tissue engineering, providing insights into the underlying mechanisms that are benefit by muscle cell alignment. The findings suggest that microstructured scaffolds are essential tools for advancing muscle tissue engineering practices.

Letztmalig verändert: 24.10.2024, 07:42:46