Combining live imaging and protein binders to study vascular branching morphogenesis in the zebrafish

Datum

05.10.2018

Zeit

11:00 - 12:00

Sprecher

Prof. Markus Affolter

Zugehörigkeit

University of Basel, Bio Center

Sprache

en

Hauptthema

Biologie

Andere Themen

Biologie, Medizin

Host

Dr. Nikolay Ninov

Beschreibung

Abstract How a complex vascular network is established during development and remodelled in growing embryos has been difficult to analyse in the past due to the lack of appropriate model systems allowing at the same time high resolution live imaging and genetic dissection. Such analyses are possible in the zebrafish and I will report on progress made in the field to better understand the control of cell behaviour during the branching and remodelling process. To better dissect molecular players regulating cell behaviour, we are functionalizing protein binders to manipulate proteins directly and acutely during vascular morphogenesis. In the future, these novel tools should allow to dissect biological processes in much more detail in living organisms. 5 most recent papers Junction-based lamellipodia drive endothelial cell rearrangements in vivo via a VE-cadherin-F-actin based oscillatory cell-cell interaction. Paatero I, Sauteur L, Lee M, Lagendijk AK, Heutschi D, Wiesner C, Guzmán C, Bieli D, Hogan BM, Affolter M, Belting HG. Nat Commun. 2018 Aug 31;9(1):3545. Protein binders and their applications in developmental biology. Harmansa S, Affolter M. Development. 2018 Jan 26;145(2). Cell behaviors and dynamics during angiogenesis. Betz C, Lenard A, Belting HG, Affolter M. Development. 2016 Jul 1;143(13):2249-60. Endothelial cell self-fusion during vascular pruning. Lenard A, Daetwyler S, Betz C, Ellertsdottir E, Belting HG, Huisken J, Affolter M. PLoS Biol. 2015 Apr 17;13(4):e1002126. In vivo analysis reveals a highly stereotypic morphogenetic pathway of vascular anastomosis. Lenard A, Ellertsdottir E, Herwig L, Krudewig A, Sauteur L, Belting HG, Affolter M. Dev Cell. 2013 Jun 10;25(5):492-506.

Links

• to announcement of the organizer

Letztmalig verändert: 05.10.2018, 02:05:38