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UID:DSC-11268
DTSTART;TZID=Europe/Berlin:20160429T130000
SEQUENCE:1461917007
TRANSP:OPAQUE
DTEND;TZID=Europe/Berlin:20160429T140000
URL:https://www.dresden-science-calendar.de/calendar/de/detail/11268
LOCATION:IFW\, Helmholtzstraße 2001069 Dresden
SUMMARY:Schön: Modeling the embryo-maternal contact zone in vitro: Differe
 ntiated long-term cultures derived from the oviduct epithelium 
CLASS:PUBLIC
DESCRIPTION:Speaker: Dr. Jennifer Schön\nInstitute of Speaker: Leibniz Ins
 titute for Farm Animal Biology (FBN)\nTopics:\nMaterialien\, Physik\n Loca
 tion:\n  Name: IFW (D2E.27\, IFW Dresden)\n  Street: Helmholtzstraße 20\n
   City: 01069 Dresden\n  Phone: \n  Fax: \nDescription: The oviduct (i.e. 
 fallopian tube in primates) is a fascinating\, multi-functional organ. It 
 hosts processes like maturation and transport of the female and male gamet
 es\, provision of the ideal milieu for fertilization and embryonic develop
 ment\, and transport of the early embryo to the uterus. The fine-tuned\, s
 ynchronized course of (inter-)actions facilitating the development of a ma
 ture\, implantable embryo in vivo is still far from being understood. Ther
 efore\, well characterized cell culture systems\, especially for the lumin
 al epithelium\, are indispensable for modern reproductive science. However
 \, the complex morphology of the epithelial layer is hard to mimic in vitr
 o. Only two culture methods preserve epithelial differentiation for more t
 han 24h: Three-dimensional organoid cultures and compartmentalized culture
  systems using porous filter supports. The compartmentalized system can ei
 ther be used to grow cells at the interface of two different media or to g
 row the cells at the Air-Liquid-Interface (ALI) with no medium in the apic
 al compartment. Primarily known from differentiated long-term cultures of 
 skin and airway epithelia\, ALI systems also proved useful to maintain dif
 ferentiation of female reproductive tract epithelia. Our group established
  highly differentiated primary ALI long-term cultures of the porcine ovidu
 ct epithelium and developed a protocol for hormonal simulation of differen
 t estrous cycle stages. Mimicking the estrus cycle in vitro allowed analys
 is of the effect of E2 and P4 on differentiation\, gene expression\, and c
 ellular function in the oviduct epithelium under defined\, standardized co
 nditions. These novel culture systems provide an innovative platform to in
 vestigate the interactions of gametes and embryos with the epithelial cell
 s of the oviduct and to elucidate the metabolic and endocrine mechanisms t
 hat influence these interactions.
DTSTAMP:20260506T221613Z
CREATED:20160428T080341Z
LAST-MODIFIED:20160429T080327Z
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